Systems Integration: Robotic In Vitro Systems

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Load new plates into incubator.

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Remove plate from incubator, transfer to Hood 1 and remove lid

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Move plate to coating station, release

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Coat plate, inner wells with 0.2 ml collagen

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Regrip plate and replace lid

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Allow plate to dry in Hood 1 tower for 4-5 hours

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Remove coated plate from Hood 1, transfer to Hood 2 and remove lid

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Place plate under camera, view/locate inserts

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Place plate under dispenser; dispense 2.0 ml SFM into outer wells

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Replace plate lid, transfer plate to incubator; incubate for 2 hours

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Remove plate from incubator, transfer to Hood 2 and remove lid

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Place plate in dispenser, dispense 0.5 ml of SFM into inner wells

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Replace plate lid, return plate to incubator; incubate for 1 hour

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Remove plate from incubator, transfer to Hood 2 and remove lid

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Place plate under camera, view/locate inserts

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Place plate under aspirator, aspirate media from outer wells

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Place plate under dispenser, dispense 2.0 ml MWS into outer wells

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Replace plate lid, place in incubator for at least 30 minutes

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After last plate is conditioned begin seeding

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Cell suspension is maintained @ 37°C, then shaken gently with 5% CO2 to maintain proper pH and keep cells in suspension.

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Remove plate from incubator, transfer to Hood 2 and remove lid

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Place plate under aspirator, aspirate from inner well

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Replace plate lid, transfer plate to Hood 1 and remove lid

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Place plate into seeding station, seed plate with 0.5 ml into inner well

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Replace plate lid, return plate to incubator; incubate for 48 hours

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Re-feed (see feeding below)

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Remove plate from incubator after 48-hour incubation

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Regrip plate, bring into Hood 2 and remove lid

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Place plate under camera to view/locate inserts

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Place plate under aspirator, aspirate media from outer wells, inner wells

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Move plate to dispenser; dispense 0.5 ml MWS into inner wells and 2.0 ml MWS into outer well

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Replace plate lid, return plate to incubator and incubate for 48 hours

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Re-feed after 48 hours for a total of 21 days